Title | Reverse Chemical Genetics [electronic resource] : Methods and Protocols / edited by Hisashi Koga |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2009 |
Connect to | http://dx.doi.org/10.1007/978-1-60761-232-2 |
Descript | online resource |
The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics -- ORFeome Cloning -- Systematic Cloning of an ORFeome Using the Gateway System -- High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins -- Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study -- High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data -- Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology -- Modification of cDNA Resources Utilized for Reverse Chemical Genetics -- High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources -- An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources -- Recombinant Protein Production by a Kaiko–Baculovirus System -- Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins -- The Refinement of Established Methods by Recent Innovations -- Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine -- Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis -- Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos -- Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins -- Potential Applicable Techniques for Reverse Chemical Genetics -- Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases -- Preparation of Highly Sensitive Protein Array Using Reactive Polymer -- High-Throughput SPR Biosensor -- Drug Discovery Through Functional Screening in the Drosophila Heart -- Permeable Cell Assay: A Method for High-Throughput Measurement of Cellular ATP Synthetic Activity -- Novel Methodology for Immobilization of Biomolecules on the Surface of a Photoresponsible Polymer Containing Azobenzene Moiety