Title | Proteomics [electronic resource] : Methods and Protocols / edited by Jörg Reinders, Albert Sickmann |
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Imprint | Totowa, NJ : Humana Press, 2009 |
Connect to | http://dx.doi.org/10.1007/978-1-60761-157-8 |
Descript | XII, 441 p. online resource |
to Proteomics -- Electrophorestic Separations -- High-Resolution Two-Dimensional Electrophoresis -- Non-classical 2-D Electrophoresis -- Protein Detection and Quantitation Technologies for Gel-Based Proteome Analysis -- Mass Spectrometry and Tandem Mass Spectrometry Applications -- MALDI MS -- Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery -- A Newcomer’s Guide to Nano-Liquid-Chromatography of Peptides -- Multidimensional Protein Identification Technology -- Characterization of Platelet Proteins Using Peptide Centric Proteomics -- Identification of the Molecular Composition of the 20S Proteasome of Mouse Intestine by High-Resolution Mass Spectrometric Proteome Analysis -- Quantitative Proteomics -- Liquid Chromatography–Mass Spectrometry-Based Quantitative Proteomics -- iTRAQ-Labeling of In-Gel Digested Proteins for Relative Quantification -- Electrospray Mass Spectrometry for Quantitative Plasma Proteome Analysis -- Interpretation of Mass Spectrometry Data -- Algorithms and Databases -- Shotgun Protein Identification and Quantification by Mass Spectrometry -- Analysis of Protein Modifications -- Proteomics Identification of Oxidatively Modified Proteins in Bra -- Isotope-Labeling and Affinity Enrichment of Phosphopeptides for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry -- Subcellular Proteomics -- VII Subcellular Proteomics Organelle Proteomics: Reduction of Sample Complexity by Enzymatic In-Gel Selection of Native Proteins -- Isolation of Plasma Membranes from the Nervous System by Countercurrent Distribution in Aqueous Polymer Two-Phase Systems -- Enrichment and Preparation of Plasma Membrane Proteins from Arabidopsis thaliana for Global Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry -- Analysis of Protein Interactions -- Tandem Affinity Purification of Protein Complexes from Mammalian Cells by the Strep/FLAG (SF)-TAP Tag -- Sequential Peptide Affinity Purification System for the Systematic Isolation and Identification of Protein Complexes from Escherichia coli -- Bioinformatical Approaches to Detect and Analyze Protein Interactions