Title | Zebrafish [electronic resource] : Methods and Protocols / edited by Graham J. Lieschke, Andrew C. Oates, Koichi Kawakami |
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Imprint | Totowa, NJ : Humana Press, 2009 |
Connect to | http://dx.doi.org/10.1007/978-1-60327-977-2 |
Descript | XI, 335 p. online resource |
Mutagenesis and Mutants -- Highly Efficient ENU Mutagenesis in Zebrafish -- Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish -- Undertaking a Successful Gynogenetic Haploid Screen in Zebrafish -- Cryopreservation and In Vitro Fertilization at the Zebrafish International Resource Center -- Transgenesis -- Transient and Stable Transgenesis Using Tol2 Transposon Vectors -- Analysis of Genes and Genome by the Tol2-Mediated Gene and Enhancer Trap Methods -- Bacterial Artificial Chromosome Transgenesis for Zebrafish -- Simple and Efficient Transgenesis with Meganuclease Constructs in Zebrafish -- Tissue-Specific Manipulations -- Nitroreductase-Mediated Cell Ablation in Transgenic Zebrafish Embryos -- Focal Electroporation in Zebrafish Embryos and Larvae -- Tissue Micromanipulation in Zebrafish Embryos -- Analyzing Gene Expression -- Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments. Part I: Array Printing and Hybridization -- Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments: Data Acquisition and Analysis -- Validating microRNA Target Transcripts Using Zebrafish Assays -- Imaging -- Multiple Embryo Time-Lapse Imaging of Zebrafish Development -- Live Cell Imaging of Zebrafish Leukocytes -- Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy -- Morphological Analysis of the Zebrafish Digestive System -- In Toto Imaging of Embryogenesis with Confocal Time-Lapse Microscopy