Title | The Nuclear Receptor Superfamily [electronic resource] : Methods and Protocols / edited by Iain J. McEwan |
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Imprint | Totowa, NJ : Humana Press, 2009 |
Connect to | http://dx.doi.org/10.1007/978-1-60327-575-0 |
Descript | 240 p. 43 illus., 4 illus. in color. online resource |
Nuclear Receptors: One Big Family -- Ligand Binding and Nuclear Receptor Turnover -- Methods for Measuring Ligand Dissociation and Nuclear Receptor Turnover in Whole Cells -- Flow Cytometry as a Tool for Measurement of Steroid Hormone Receptor Protein Expression in Leukocytes -- X-Ray Crystallography of Agonist/Antagonist-Bound Receptors -- Nuclear Localization and DNA Binding -- FRAP and FRET Methods to Study Nuclear Receptors in Living Cells -- Receptor-DNA Interactions: EMSA and Footprinting -- Chromatin Immunoprecipitation (ChIP) Methodology and Readouts -- Nuclear Receptor—Co-Regulatory Protein Interactions -- Yeast-Based Reporter Assays for the Functional Characterization of Cochaperone Interactions with Steroid Hormone Receptors -- High Throughput Analysis of Nuclear Receptor–Cofactor Interactions -- Binding Affinity and Kinetic Analysis of Nuclear Receptor/Co-Regulator Interactions Using Surface Plasmon Resonance -- Using RNA Interference to Study Protein Function -- Using Intrinsic Fluorescence Emission Spectroscopy to Study Steroid Receptor and Coactivator Protein Conformation Dynamics -- Pathophysiological Analysis of Nuclear Receptor Function -- Development of Phosphorylation Site-Specific Antibodies to Nuclear Receptors -- Tissue-Selective Knockouts of Steroid Receptors: A Novel Paradigm in the Study of Steroid Action -- Methods for Identifying and Studying Genetic Alterations in Hormone-Dependent Cancers