Title | Histology Protocols [electronic resource] / edited by Tim D. Hewitson, Ian A. Darby |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2010 |
Connect to | http://dx.doi.org/10.1007/978-1-60327-345-9 |
Descript | X, 230 p. online resource |
Tissue Processing -- Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy -- An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue -- Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Renal Glomerulus -- Staining Techniques -- Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells -- Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems -- Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Brain Sections -- High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy -- Lectin Histochemistry for Light and Electron Microscopy -- Duplex In Situ Hybridization in the Study of Gene Co-regulation in the Vertebrate Brain -- Special Stains for Extracellular Matrix -- Active Staining of Mouse Embryos for Magnetic Resonance Microscopy -- Immunohistochemical Detection of Tumour Hypoxia -- In Situ Localization of Apoptosis Using TUNEL -- Imaging Techniques -- Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord -- High-Resolution Confocal Imaging in Tissue -- Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicrography -- Image Analysis and Quantitative Morphology