Title | Membrane Proteomics [electronic resource] : Methods and Protocols / edited by Matthew J. Peirce, Robin Wait |
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Imprint | Totowa, NJ : Humana Press, 2009 |
Connect to | http://dx.doi.org/10.1007/978-1-60327-310-7 |
Descript | XIII, 342 p. online resource |
In Silico Methods for Prediction of Membrane Protein Hydrophobicity and Topology -- Online Tools for Predicting Integral Membrane Proteins -- In Silico Identification of Novel G Protein Coupled Receptors -- Transcriptome-Based Identification of Candidate Membrane Proteins -- Extraction and Purification of Membrane Proteins -- Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS-PAGE Two-Dimensional Electrophoresis -- Extraction of Yeast Mitochondrial Membrane Proteins by Solubilization and Detergent/Polymer Aqueous Two-Phase Partitioning -- 16-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electrophoresis -- Sequential Detergent Extraction Prior to Mass Spectrometry Analysis -- Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning -- Protocol to Enrich and Analyze Plasma Membrane Proteins -- Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing -- Identification of Target Membrane Proteins as Detected by Phage Antibodies -- Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease Substrates -- Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Membrane Subtraction -- Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics -- Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identification -- Isolation of Extracellular Membranous Vesicles for Proteomic Analysis -- Separation of Membrane Proteins -- Enrichment of Human Platelet Membranes for Proteomic Analysis -- Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis -- Two-Dimensional Separation of Membrane Proteins by 16-BAC-SDS-PAGE -- Identification and Quantification of Membrane Proteins -- MudPIT Analysis: Application to Human Heart Tissue -- Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis -- Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry -- Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins