Title | Antibody Phage Display [electronic resource] : Methods and Protocols / edited by Robert Aitken |
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Imprint | Totowa, NJ : Humana Press, 2009 |
Connect to | http://dx.doi.org/10.1007/978-1-60327-302-2 |
Descript | X, 240 p. 22 illus. online resource |
Antibody Phage Display: Overview of a Powerful Technology that Has Quickly Translated to the Clinic -- Design and Construction of Synthetic Phage-Displayed Fab Libraries -- Affinity Isolation of Antigen-Specific Circulating B Cells for Generation of Phage Display-Derived Human Monoclonal Antibodies -- Construction of Phage Antibody Repertoires from the Blood of West Nile Virus-Infected Donors -- Anti-?2GP-I and Anti-Prothrombin Antibodies Generated by Phage Display -- The Generation and Selection of Single-Domain, V Region Libraries from Nurse Sharks -- The Isolation of scFvs Against Small Target Molecules -- Display and Selection of scFv Antibodies on HEK-293T Cells -- Isolation of scFvs that Inhibit the NS3 Protease of Hepatitis C Virus by a Combination of Phage Display and a Bacterial Genetic Screen -- Guided Selection Methods Through Chain Shuffling -- Sequential Antigen Panning for Selection of Broadly Cross-Reactive HIV-1-Neutralizing Human Monoclonal Antibodies -- Selection of Antibodies Able to Rapidly Enter Mammalian Cells -- Identification of the Specificity of Isolated Phage Display Single-Chain Antibodies Using Yeast Two-Hybrid Screens -- Generation of Bispecific and Tandem Diabodies -- Intrabody Expression in Eukaryotic Cells -- High-Level Periplasmic Expression and Purificationof scFvs -- Expression of Single-Chain Fv Fragments in E. coli Cytoplasm -- High-Level Expression of a Phage Display-Derived scFv in Pichia pastoris