Title | Characterization of Nanoparticles Intended for Drug Delivery [electronic resource] / edited by Scott E. McNeil |
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Imprint | Totowa, NJ : Humana Press, 2011 |
Edition | 1 |
Connect to | http://dx.doi.org/10.1007/978-1-60327-198-1 |
Descript | XII, 269 p. online resource |
Unique Benefits of Nanotechnology to Drug Delivery and Diagnostics -- Challenges for Nanoparticle Characterization -- Considerations when submitting nano-therapeutics to FDA/CDER for regulatory review -- Measuring the Hydrodynamic Size of Nanoparticles in Aqueous Media Using Batch-Mode Dynamic Light Scattering -- Characterization of Nanoparticles by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry -- Zeta Potential Measurement -- Size Measurement of Nanoparticles using Atomic Force Microscopy -- Biological Tissue and Cell Culture Specimen Preparation for TEM Nanoparticle Characterization.– SEM X-ray Microanalysis of Nanoparticles Present in Tissue or Cultured Cell Thin Sections -- Detecting and Measuring Free Gadolinium in Nanoparticles for MRI Imaging -- Lipid Component Quantitation by Thin Layer Chromatography -- Detection and Quantitative Evaluation of Endotoxin Contamination in Nanoparticle Formulations by LAL-based Assays -- Analysis of microbial contamination in nanoparticle formulations -- Gold Nanoparticle Quantitation via Fluorescence in Solution and Cell Culture -- Quantitation of Nanoparticles in Serum Matrix by Capillary Electrophoresis -- Evaluation of Cytotoxicity of Nanoparticulate Materials in Porcine Kidney Cells and Human Hepatocarcinoma Cells -- Monitoring Nanoparticle-Treated Hepatacarcinoma Cells for Apoptosis – Detecting Reactive Oxygen Species in Primary Hepatocytes Treated with Nanoparticles -- Assay to Detect Lipid Peroxidation Upon Exposure to Nanoparticles -- Monitoring Glutathione Homeostasis in Nanoparticle-Treated Hepatocytes -- Autophagy Monitoring Assay: Qualitative Analysis of MAP LC3-I to II Conversion by Immunoblot -- Monitoring Lysosomal Activity in Nanoparticle-Treated Cells -- Method for Analysis of Nanoparticle Hemolytic Properties in Vitro -- Method for In Vitro Analysis of Nanoparticle Thrombogenic Properties -- Qualitative Analysis of Total Complement Activation by Nanoparticles -- Method for Analysis of Nanoparticle Effects on Cellular Chemotaxis -- In Vitro Analysis of Nanoparticle Uptake by Macrophages Using Chemiliuminescence