Title | Bacteriophages [electronic resource] : Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions / edited by Martha R.J. Clokie, Andrew M. Kropinski |
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Imprint | Totowa, NJ : Humana Press, 2009 |
Connect to | http://dx.doi.org/10.1007/978-1-60327-164-6 |
Descript | XXII, 308 p. 41 illus. online resource |
Bacteriophage Isolation -- Methods for the Isolation of Viruses from Environmental Samples -- Bacteriophage Enrichment from Water and Soil -- Isolation of Phage via Induction of Lysogens -- Isolation of Cyanophages from Aquatic Environments -- Isolation of Viruses from High Temperature Environments -- Isolation of Novel Large and Aggregating Bacteriophages -- Bacteriophage Characterization -- Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay -- Enumeration of Bacteriophages by the Direct Plating Plaque Assay -- Enumeration of Bacteriophages Using the Small Drop Plaque Assay System -- Determination of Virus Abundance by Epifluorescence Microscopy -- Enumeration of Bacteriophages Using Flow Cytometry -- Basic Phage Electron Microscopy -- Phage Classification and Characterization -- Phage Host Range and Efficiency of Plating -- Measurement of the Rate of Attachment of Bacteriophage to Cells -- Measurement of the Bacteriophage Inactivation Kinetics with Purified Receptors -- Bacteriophage Plaques: Theory and Analysis -- Practical Methods for Determining Phage Growth Parameters -- Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes -- Bacteriophage-Host Interactions -- Construction of Phage Mutants -- Modifying Bacteriophage with Recombineering -- Identification and Isolation of Lysogens with Induced Prophage -- Generalized Transduction -- Preparation and Characterization of Anti-phage Serum -- Generalized Transduction by Lytic Bacteriophages