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TitleNeuropeptide Techniques [electronic resource]
Author edited by Illana Gozes
ImprintTotowa, NJ : Humana Press, 2008
Connect tohttp://dx.doi.org/10.1007/978-1-60327-099-1
Descript XII, 160 p. 52 illus., 1 illus. in color. online resource

SUMMARY

Major advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function. With a selected description of techniques in neuropeptide research, Neuropeptide Methods covers aspects of design, synthesis and biological evaluation of peptides and peptide analogues. Written by leaders in the field, this book explains primary cell cultures and cell lines, as well as techniques in neuropeptide processing, trafficking and secretion. In vivo techniques quantifying blood-brain barrier permeability to small proteins in mice and developmental milestones of behavior in newborn mice are described. Neuropeptide Methods provides the reader with broad perspectives and breadth of knowledge on current topics related to neuropeptide research


CONTENT

A Short Introduction -- Preface -- Contributors -- The Design, Synthesis, and Biological Evaluation of VIP and VIP Analogs David Dangoor, Sara Rubinraut, Mati Fridkin, and Illana Gozes -- Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example Illana Gozes, Miri Holtser-Cochav, and Karin Vered -- Primary Cell Cultures and Cell Lines Inna Divinski, Inbar Pilzer, and Illana Gozes -- Transfection of DNA into Cells Inbar Pilzer, Inna Divinski, and Illana Gozes -- Transgenic Delivery and Detection of GFP in Neuropeptide Neurons J.L. Holter, J.S. Davies, P.-S. Man, T. Wells, and D.A. Carter -- Integrative Technologies for Neuropeptide Characterization Sergey E. Ilyin and Carlos R. Plata-Salamán -- Techniques in Neuropeptide Processing, Trafficking, and Secretion Niamh X. Cawley, Tulin Yanik, Irina Arnaoutova,Hong Lou, Nimesh Patel, and Y. Peng Loh -- In vivo Techniques Quantifying Blood–Brain Barrier Permeability to Small Proteins in Mice Weihong Pan, and Abba J. Kastin -- Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs Terry W. Moody, Michael Schumann, and Robert T. Jensen -- Developmental Milestones in the Newborn Mouse Joanna M. Hill, Maria A. Lim, and Madeleine M. Stone


Medicine Human physiology Neurosciences Proteomics Proteins Neurobiology Biomedicine Neurosciences Proteomics Protein Science Human Physiology Neurobiology



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