Title | Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols [electronic resource] / edited by Andreas Marx, Oliver Seitz |
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Imprint | Totowa, NJ : Humana Press, 2008 |
Connect to | http://dx.doi.org/10.1007/978-1-60327-040-3 |
Descript | XII, 266 p. 77 illus., 17 illus. in color. online resource |
Basic Principles of Fluorescence and Energy Transfer -- Applications -- Multiplex Detection of Mutations -- Detection of Salmonella Species in Foodstuffs -- Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR -- Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma -- Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan® Real-time PCR and RT-PCR -- High-Throughput Real-Time PCR -- Design and Use of Scorpions Fluorescent Signaling Molecules -- Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification -- New Technologies -- EasyBeacons™ for the Detection of Methylation Status of Single CpG Duplets -- Quenched Autoligation Probes -- HyBeacon® Probes for Rapid DNA Sequence Detection and Allele Discrimination -- FIT-Probes in Real-Time PCR -- SNP Genotyping by Unlabeled Probe Melting Analysis -- Non-Nucleic Acid Targets -- Molecular Beacons for Protein—DNA Interaction Studies -- DNA Polymerase Profiling -- Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions -- Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes