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TitleAvidin-Biotin Interactions [electronic resource] : Methods and Applications
Author edited by Robert J. McMahon
ImprintTotowa, NJ : Humana Press, 2008
Connect tohttp://dx.doi.org/10.1007/978-1-59745-579-4
Descript XIV, 232 p. 38 illus. online resource

SUMMARY

For researchers today, the avidin-biotin interaction is among the most widely exploited among biomedical scientists. Despite its popularity, avidin-biotin methodology suffers from a lack of clarity associated with popular, low cost, prefabricated kits used by many in the field. In Avidin-Biotin Interactions: Methods and Applications, leaders in avidin-biotin methodology share their knowledge relating to the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. Although avidin-biotin based kits based have been produced for quite some time and remain a widely used methodology, many of the chapters in this text describe situations in which the previous methodology failed due to some factor, and the authors developed new and better ways for the avidin-biotin system to be utilized. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist. Each chapter in Avidin-Biotin Interactions: Methods and Applications contains not only excellent descriptions of protocols for the lab but also the experience of researchers who used specific methodology, found a problem with that methodology in a new setting, and ultimately devised ways to minimize or obviate the limitations of the technology


CONTENT

Preparation of Avidin Conjugates -- Coupling of Antibodies With Biotin -- Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin -- Use of the Avidin (Imino)Biotin System as a General Approach to Affinity Precipitation -- Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis -- Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS–PAGE Using Biotin-4-Fluorescein -- Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives -- Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins -- High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli -- Endogenous Biotin in Rat Brain -- Pseudo-Immunolabelling With the Avidin–Biotin–Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina -- Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones -- Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay -- Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin -- Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure -- A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin -- Biotin–Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications


Life sciences Immunology Biotechnology Biochemistry Proteins Cell biology Life Sciences Protein Science Cell Biology Biochemistry general Biotechnology Immunology



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