Title | Avidin-Biotin Interactions [electronic resource] : Methods and Applications / edited by Robert J. McMahon |
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Imprint | Totowa, NJ : Humana Press, 2008 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-579-4 |
Descript | XIV, 232 p. 38 illus. online resource |
Preparation of Avidin Conjugates -- Coupling of Antibodies With Biotin -- Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin -- Use of the Avidin (Imino)Biotin System as a General Approach to Affinity Precipitation -- Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis -- Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS–PAGE Using Biotin-4-Fluorescein -- Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives -- Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins -- High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli -- Endogenous Biotin in Rat Brain -- Pseudo-Immunolabelling With the Avidin–Biotin–Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina -- Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones -- Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay -- Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin -- Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure -- A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin -- Biotin–Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications