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TitlePatch-Clamp Methods and Protocols [electronic resource]
Author edited by Peter Molnar, James J. Hickman
ImprintTotowa, NJ : Humana Press, 2007
Connect tohttp://dx.doi.org/10.1007/978-1-59745-529-9
Descript XI, 321 p. 71 illus. online resource

SUMMARY

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is divided into three sections that cover the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step by step instructions on how to use whole-cell and single-channel patch clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies


CONTENT

Pharmacology -- Pharmacological Analysis of Recombinant NR1a/2A and NR1a/2B NMDA Receptors Using the Whole-Cell Patch-Clamp Method -- Memantine as an Example of a Fast, Voltage-Dependent, Open Channel N-Methyl-d-Aspartate Receptor Blocker -- Methods for Evaluation of Positive Allosteric Modulators of Glutamate AMPA Receptors -- Automated Voltage-Clamp Technique -- Flip-the-Tip -- The Roboocyte -- Physiology -- Infrared-Guided Laser Stimulation as a Tool for Elucidating the Synaptic Site of Expression of Long-Term Synaptic Plasticity -- Single-Cell RT–PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity -- Mechanosensitive Ion Channels Investigated Simultaneously by Scanning Probe Microscopy and Patch Clamp -- Synaptic Connectivity in Engineered Neuronal Networks -- Modeling of Action Potential Generation in NG108-15 Cells -- Whole-Cell Voltage Clamp on Skeletal Muscle Fibers With the Silicone-Clamp Technique -- Determination of Channel Properties at the Unitary Level in Adult Mammalian Isolated Cardiomyocytes -- Electrophysiological Properties of Embryonic Stem Cells During Differentiation Into Cardiomyocyte-Like Cell Types -- Hybrid Neuronal Network Studies Under Dynamic Clamp -- Cardiac Channelopathies Studied With the Dynamic Action Potential-Clamp Technique -- Biophysics -- Principles of Single-Channel Kinetic Analysis -- Use of Xenopus Oocytes to Measure Ionic Selectivity of Pore-Forming Peptides and Ion Channels -- Estimation of Quantal Parameters With Multiple-Probability Fluctuation Analysis


Life sciences Human physiology Neurosciences Pharmacology Biochemistry Biophysics Biological physics Life Sciences Biochemistry general Human Physiology Pharmacology/Toxicology Biophysics and Biological Physics Neurosciences



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