Title | DNA Methylation [electronic resource] : Methods and Protocols / edited by Jörg Tost |
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Imprint | Totowa, NJ : Humana Press, 2009 |
Edition | 2 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-522-0 |
Descript | XVI, 392 p. online resource |
DNA Methylation: An Introduction to the Biology and the Disease-Associated Changes of a Promising Biomarker -- Global Methylation Levels -- Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometry -- Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels -- Methods For Whole Genome Analysis Of Dna Methylation Patterns -- Immunodetection Array -- Methylated DNA Immunoprecipitation (MeDIP) -- The MIRA Method for DNA Methylation Analysis -- The Help Assay -- Differential Methylation Hybridization: Profiling DNA Methylation with a High-Density CpG Island Microarray -- Analysis of DNA Methylation by Amplification of Intermethylated Sites (AIMS) -- Methylation-Sensitive Representational Difference Analysis (MS-RDA) -- Restriction Landmark Genomic Scanning: Analysis of CpG Islands in Genomes by 2D Gel Electrophoresis -- GoldenGate® Assay for DNA Methylation Profiling -- 5?-Azacytidine Expression Arrays -- Gene-Specific Methylation Analysis -- DNA Methylation Analysis by Bisulfite Conversion, Cloning, and Sequencing of Individual Clones -- Identification and Quantification of Differentially Methylated Loci by the Pyrosequencing™ Technology -- Mass Spectrometric Analysis of Cytosine Methylation by Base-Specific Cleavage and Primer Extension Methods -- Melting Curve Assays for DNA Methylation Analysis -- Methylation SNaPshot: A Method for the Quantification of Site-Specific DNA Methylation Levels -- Bio-COBRA: Absolute Quantification of DNA Methylation in Electrofluidics Chips -- Restriction Digestion and Real-Time PCR (qAMP) -- MethylQuant: A Real-Time PCR-Based Method to Quantify DNA Methylation at Single Specific Cytosines -- Methylation-Specific PCR -- MethyLight -- Quantification of Methylated DNA by HeavyMethyl Duplex PCR -- Special Applications -- Analysis of Methylated Circulating DNA in Cancer Patients’ Blood -- Prevention of PCR Cross-Contamination by UNG Treatment of Bisulfite-Treated DNA -- Profiling DNA Methylation from Small Amounts of Genomic DNA Starting Material: Efficient Sodium Bisulfite Conversion and Subsequent Whole-Genome Amplification