Title | Mitochondrial DNA [electronic resource] : Methods and Protocols / edited by Jeffrey A. Stuart |
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Imprint | Totowa, NJ : Humana Press, 2009 |
Edition | 2 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-521-3 |
Descript | XII, 404 p. 74 illus., 1 illus. in color. online resource |
Mitochondrial DNA Replication, Transcription, Translation, and Respiratory Complex Assembly -- Biochemical Isolation of mtDNA Nucleoids from Animal Cells -- Analysis of Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis -- Comparative Purification Strategies for Drosophila and Human Mitochondrial DNA Replication Proteins: DNA Polymerase ? and Mitochondrial Single-Stranded DNA-Binding Protein -- Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease -- Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein -- Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA -- Inducible Expression in Human Cells, Purification, and In Vitro Assays for the Mitochondrial DNA Helicase Twinkle -- Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase -- Methods for Studying Mitochondrial Transcription Termination with Isolated Components -- Oxidative Phosphorylation: Synthesis of Mitochondrially Encoded Proteins and Assembly of Individual Structural Subunits into Functional Holoenzyme Complexes -- Mitochondrial DNA Damage and Repair -- Reactive Oxygen Species Production by Mitochondria -- Measuring mtDNA Damage Using a Supercoiling-Sensitive qPCR Approach -- Quantitative Analysis of Oxidized Guanine, 8-Oxoguanine, in Mitochondrial DNA by Immunofluorescence Method -- In Vitro Measurement of DNA Base Excision Repair in Isolated Mitochondria -- Targeting Repair Proteins to the Mitochondria of Mammalian Cells Through Stable Transfection, Transient Transfection, Viral Transduction, and TAT-Mediated Protein Transduction -- Construction and Characterization of a Cell Line Deficient in Repair of Mitochondrial, but Not Nuclear, Oxidative DNA Damage -- Mitochondrial DNA Mutations -- Mitochondrial DNA Oxidative Damage and Mutagenesis in Saccharomyces cerevisiae -- Determination of DNA Mutation Load in Human Tissues Using Denaturing HPLC-Based Heteroduplex Analysis -- Rapid Identification of Unknown Heteroplasmic Mitochondrial DNA Mutations with Mismatch-Specific Surveyor Nuclease -- Collection of Isolated Cells for Studying Mitochondrial DNA Mutations Within Individual Cells -- Quantitative Analysis of Somatic Mitochondrial DNA Mutations by Single-Cell Single-Molecule PCR -- Measuring DNA Precursor Pools in Mitochondria -- Establishment of Human Cell Lines Lacking Mitochondrial DNA