Title | Gene Knockout Protocols [electronic resource] : Second Edition / edited by Wolfgang Wurst, Ralf Kühn |
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Imprint | Totowa, NJ : Humana Press, 2009 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-471-1 |
Descript | XVI, 516 p. online resource |
Gene Modification in ES Cells -- Overview on Mouse Mutagenesis -- Construction of Gene-Targeting Vectors by Recombineering -- Gene-Trap Vectors and Mutagenesis -- Chromosome Engineering in ES Cells -- Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides -- Generation of shRNA Transgenic Mice -- Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonate -- Stem Cell Manipulation -- Gene Targeting in Mouse Embryonic Stem Cells -- Manipulating Mouse Embryonic Stem Cells -- ES Cell Line Establishment -- Generation of Double-Knockout Embryonic Stem Cells -- Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro -- Cloning of ES Cells and Mice by Nuclear Transfer -- Genetically Engineered Mice -- Isolation, Microinjection and Transfer of Mouse Blastocysts -- Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryos -- VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eight-Cell-Stage Embryos -- Generation of Cre Recombinase-Expressing Transgenic Mice Using Bacterial Artificial Chromosomes -- Inducible Cre Mice -- Creation and Use of a Cre Recombinase Transgenic Database -- Transposon Mutagenesis in Mice -- Lentiviral Transgenesis -- Sperm Cryopreservation and In Vitro Fertilization -- Phenotype Analysis -- Influence of Genetic Background on Genetically Engineered Mouse Phenotypes -- Pathologic Phenotyping of Mutant Mice -- Systemic First-Line Phenotyping