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TitleHepatitis C [electronic resource] : Methods and Protocols
Author edited by Hengli Tang
ImprintTotowa, NJ : Humana Press, 2009
Connect tohttp://dx.doi.org/10.1007/978-1-59745-394-3
Descript XXII, 466 p. 74 illus., 1 illus. in color. online resource

SUMMARY

With the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative cycle of the virus. By providing a compilation of cutting-edge research techniques currently in use in HCV labs worldwide, Hepatitis C: Methods and Protocols, Second Edition fully updates the previous edition to reflect this progress which promises to unlock the remaining vital information regarding this human pathogen and its interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and comprehensive, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease


CONTENT

Detection and Quantification of HCV -- Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays -- Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen -- Immunohistochemical Detection of HCV Proteins in Liver Tissue -- HCV Nomenclature, Genotyping, and Quasispecies -- Nomenclature and Numbering of the Hepatitis C Virus -- A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates -- Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry -- HCV Replication in Vitro -- Purification and Crystallization of NS5A Domain I of Hepatitis C Virus -- NS5A Phosphorylation and Hyperphosphorylation -- Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies -- Structural and Functional Analysis of the HCV p7 Protein -- HCV Replicons: Overview and Basic Protocols -- Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA -- Studying HCV RNA Synthesis In Vitro with Replication Complexes -- Proteomics Study of the Hepatitis C Virus Replication Complex -- Investigation of the Hepatitis C Virus Replication Complex -- Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease -- Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay -- High-Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System -- Inhibition of HCV Replication by Small Interfering RNA -- HCV Entry and Infection -- Cellular Receptors and HCV Entry -- Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp) -- Screening of Small-Molecule Compounds as Inhibitors of HCV Entry -- Isolation of JFH-1 Strain and Development of an HCV Infection System -- Measuring HCV Infectivity Produced in Cell Culture and In Vivo -- Genotype 1a HCV (H77S) Infection System -- Full-Length Infectious HCV Chimeras -- Adaptation of the Hepatitis C Virus to Cell Culture -- Primary Human Hepatocyte Culture for HCV Study -- In Vivo Study of HCV in Mice with Chimeric Human Livers -- Immunity and Modeling -- Determination of HCV-Specific T-Cell Activity -- Analysis of HCV-Specific T Cells by Flow Cytometry -- Detection of Neutralizing Antibodies with HCV Pseudoparticles (HCVpp) -- Mathematical Modeling of HCV Infection and Treatment


Medicine Virology Hepatology Infectious diseases Medicine & Public Health Hepatology Infectious Diseases Virology



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