Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995. This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources
CONTENT
Long-Term Ex Situ Conservation of Biological Resources and the Role of Biological Resource Centers -- The Principles of Freeze-Drying -- Principles of Cryopreservation -- Lyophilization of Proteins -- Vacuum-Drying and Cryopreservation of Prokaryotes -- Freeze-Drying of Yeast Cultures -- Cryopreservation of Yeast Cultures -- Freeze-Drying Fungi Using a Shelf-Freeze Drier -- Cryopreservation and Freeze-Drying of Fungi Employing Centrifugal and Shelf Freeze-Drying -- Cryopreservation of Microalgae and Cyanobacteria -- Cryopreservation of Plant Cell Suspensions -- Cryopreservation of Shoot Tips and Meristems -- Cryopreservation of Desiccation-Tolerant Seeds -- Cryopreservation of Fish Sperm -- Cryopreservation of Avian Spermatozoa -- Cryopreservation of Animal and Human Cell Lines -- Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use -- Cryopreservation of Human Embryonic Stem Cell Lines -- Cryopreservation of Primary Animal Cell Cultures -- Cryopreservation of Red Blood Cells and Platelets -- Cryopreservation of Mammalian Semen -- Cryopreservation of Mammalian Oocytes -- Cryopreservation of Mammalian Embryos
