Title | Adhesion Protein Protocols [electronic resource] / edited by Amanda S. Coutts |
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Imprint | Totowa, NJ : Humana Press, 2007 |
Edition | Second Edition |
Connect to | http://dx.doi.org/10.1007/978-1-59745-353-0 |
Descript | X, 234 p. 54 illus., 2 illus. in color. online resource |
Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells -- Separation of Cell-Cell Adhesion Complexes by Differential Centrifugation -- Analysis of Neutrophil Chemotaxis -- Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells -- Biochemical Purification of Pseudopodia from Migratory Cells -- Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture -- Quantitative Analyses of Cell Adhesion Strength -- Using RNA Interference to Knock Down the Adhesion Protein TES -- Assaying Calpain Activity -- Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray -- Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells -- Bioinformatic Analysis of Adhesion Proteins -- Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching -- Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture -- In Vitro Actin Assembly Assays and Purification From Acanthamoeba