Title | Kidney Research [electronic resource] : Experimental Protocols / edited by Gavin J. Becker, Tim D. Hewitson |
---|---|
Imprint | Totowa, NJ : Humana Press, 2009 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-352-3 |
Descript | XVI, 272 p. 62 illus., 10 illus. in color. online resource |
Isolation and Propagation of Cell Populations -- Isolation and Propagation of Glomerular Mesangial Cells -- Isolation and Primary Culture of Human Proximal Tubule Cells -- Propagation and Culture of Renal Fibroblasts -- Animal Models -- Small Animal Models of Kidney Disease: A Review -- Techniques and Applications -- Measurement of Glomerular Filtration Rate in Conscious Mice -- Laser Capture Microdissection of Kidney Tissue -- Quantitative Gene Expression Analysis in Kidney Tissues -- In Vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy -- Using In Situ Hybridisation to Localize Renal Gene Expression in Tissue Sections -- Immuno and Lectin Histochemistry for Renal Light Microscopy -- Immuno and Lectin Histochemistry for Renal Electron Microscopy -- Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia -- Identification of Apoptosis in Kidney Tissue Sections -- Cell-Populated Floating Collagen Lattices: An In Vitro Model of Parenchymal Contraction -- Mechanical Stretch-Induced Signal Transduction in Cultured Mesangial Cells -- Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue -- SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue -- In Vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli