Title | Macromolecular Crystallography Protocols [electronic resource] : Volume 1, Preparation and Crystallization of Macromolecules / edited by John M. Walker, Sylvie Doublié |
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Imprint | Totowa, NJ : Humana Press, 2007 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-209-0 |
Descript | XIV, 280 p. 49 illus. online resource |
A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag -- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project -- Baculoviral Expression of an Integral Membrane Protein for Structural Studies -- Protein Engineering -- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems -- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals -- Screening and Optimization Methods for Nonautomated Crystallization Laboratories -- Improving Marginal Crystals -- Optimization Techniques for Automation and High Throughput -- Three-Dimensional Crystallization of Membrane Proteins -- Crystallization of Protein-DNA Complexes -- Preparation and Crystallization of RNA -- Crystallization of RNA-Protein Complexes