Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure
CONTENT
A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag -- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project -- Baculoviral Expression of an Integral Membrane Protein for Structural Studies -- Protein Engineering -- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems -- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals -- Screening and Optimization Methods for Nonautomated Crystallization Laboratories -- Improving Marginal Crystals -- Optimization Techniques for Automation and High Throughput -- Three-Dimensional Crystallization of Membrane Proteins -- Crystallization of Protein-DNA Complexes -- Preparation and Crystallization of RNA -- Crystallization of RNA-Protein Complexes
