Title | RNAi [electronic resource] : Design and Application / edited by Sailen Barik |
---|---|
Imprint | Totowa, NJ : Humana Press, 2008 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-191-8 |
Descript | XIV, 270 p. 48 illus., 3 illus. in color. online resource |
Application of RNAi in Diverse Organisms -- Principles of Dicer Substrate (D-siRNA) Design and Function -- Expression, Purification, and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymes -- In vitro RNA Cleavage Assay for Argonaute-Family Proteins -- Identifying siRNA-Induced Off-Targets by Microarray Analysis -- Application of RNAi in Diverse Organisms -- Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis -- Nasal Delivery of siRNA -- RNA Interference as a Genetic Tool in Trypanosomes -- Lentivirus-Mediated RNA Interference in Mammalian Neurons -- Silencing Genes by RNA Interference in the Protozoan Parasite Entamoeba histolytica -- Use of RNAi in C. elegans -- Application of siRNA Against SARS in the Rhesus Macaque Model -- siRNA and shRNA as Anticancer Agents in a Cervical Cancer Model -- Identification and Expression Analysis of Small RNAs During Development -- Screening and Identification of Virus-Encoded RNA Silencing Suppressors -- Application of RNA Interference in Functional Genomics Studies of a Social Insect -- Temporal Control of Gene Silencing by in ovo Electroporation -- Altering Flower Color in Transgenic Plants by RNAi-Mediated Engineering of Flavonoid Biosynthetic Pathway -- Transgenic RNA Interference in Mice