Title | Protein Engineering Protocols [electronic resource] / edited by Katja M. Arndt, Kristian M. Müller |
---|---|
Imprint | Totowa, NJ : Humana Press, 2007 |
Connect to | http://dx.doi.org/10.1385/1597451878 |
Descript | 328 p. 65 illus., 1 illus. in color. online resource |
Design and Computational Strategies for Protein Engineering -- Combinatorial Protein Design Strategies Using Computational Methods -- Global Incorporation of Unnatural Amino Acids in Escherichia coli -- Considerations in the Design and Optimization of Coiled Coil Structures -- Calcium Indicators Based on Calmodulin-Fluorescent Protein Fusions -- Design and Synthesis of Artificial Zinc Finger Proteins -- Monobodies -- Engineering Site-Specific Endonucleases -- Evolutionary Strategies for Protein Engineering -- Protein Library Design and Screening -- Protein Design by Binary Patterning of Polar and Nonpolar Amino Acids -- Versatile DNA Fragmentation and Directed Evolution With Nucleotide Exchange and Excision Technology -- Degenerate Oligonucleotide Gene Shuffling -- M13 Bacteriophage Coat Proteins Engineered for Improved Phage Display -- Ribosome-Inactivation Display System -- Compartmentalized Self-Replication -- Synthesis of Degenerated Libraries of the Ras-Binding Domain of Raf and Rapid Selection of Fast-Folding and Stable Clones With the Dihydrofolate Reductase Protein Fragment Complementation Assay -- A General Method of Terminal Truncation, Evolution, and Re-Elongation to Generate Enzymes of Enhanced Stability