Title | Exocytosis and Endocytosis [electronic resource] / edited by Andrei I. Ivanov |
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Imprint | Totowa, NJ : Humana Press, 2008 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-178-9 |
Descript | XV, 412 p. online resource |
An Overview of Methods for Analyzing Vesicular Trafficking -- Vesicular Trafficking: Molecular Tools and Targets -- Pharmacological Inhibition of Endocytic Pathways: Is It Specific Enough to Be Useful? -- Cell-Free and Biochemical Assays of Exocytosis and Endocytosis -- In Vitro Assays to Measure SNARE-Mediated Vesicle Fusion -- Imaging Ca2+-Triggered Exocytosis of Single Secretory Granules on Plasma Membrane Lawns from Neuroendocrine Cells -- FRAP Analysis of Secretory Granule Lipids and Proteins in the Sea Urchin Egg -- A Cell-Free Assay for Endocytosis of E-Cadherin -- Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin -- Fractionation of Subcellular Membrane Vesicles of Epithelial and Nonepithelial Cells by OptiPrep™ Density Gradient Ultracentrifugation -- Identification and Characterization of Interacting Partners of Rab GTPases by Yeast Two-Hybrid Analyses -- Analysis of Endocytosis and Exocytosis in Different Cell Systems -- High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins -- Studying Phagocytosis by Live-Cell Scintillation Proximity Assay -- Transcytosis of Polymeric Immunoglobulin A in Polarized Madin–Darby Canine Kidney Cells -- The Use of Syntaxin Chimeras to Study Polarized Protein Trafficking in Epithelial Cells -- Targeting the Epithelial SNARE Machinery by Bacterial Neurotoxins -- Exocytosis of Endothelial Cells Is Regulated by N-Ethylmaleimide-Sensitive Factor -- Molecular Dissection of HCl Secretion in Gastric Parietal Cells Using Streptolysin O Permeabilization -- Addressing Membrane Protein Topology Using the Fluorescence Protease Protection (FPP) Assay -- Visualizing Clathrin-Mediated IgE Receptor Internalization by Electron and Atomic Force Microscopy -- Analyzing Endosomes in Nonsectioned Cells by Transmission Electron Microscopy -- Imaging Exocytosis of Single Insulin Secretory Granules With TIRF Microscopy -- Reconstitution of Depolarization and Ca2+-Evoked Secretion in Xenopus Oocytes Monitored by Membrane Capacitance -- Low-Noise Recording of Single-Vesicle Capacitance Steps in Cell-Attached Patches -- Good Practices in Single-Cell Amperometry -- Analysis of Single-Vesicle Exocytotic Events Recorded by Amperometry -- Analysis of Exocytosis and Endocytosis in Model Organisms -- Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in Caenorhabditis elegans -- FM 1-43 Labeling of Synaptic Vesicle Pools at the Drosophila Neuromuscular Junction -- Probing E-Cadherin Endocytosis by Morpholino-Mediated Rab5 Knockdown in Zebrafish -- Quantifying Endocytosis In Vivo Using Intravital Two-Photon Microscopy