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TitleAutophagosome and Phagosome [electronic resource]
Author edited by Vojo Deretic
ImprintTotowa, NJ : Humana Press, 2008
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Descript XVII, 455 p. 97 illus., 1 illus. in color. online resource


Autophagy and phagocytosis are distinct yet partially morphologically similar processes. In Autophagosome and Phagosome, authoritative scientists present easy-to-follow methods on autophagy, a rapidly growing field with a need for standards of assessment, and phagocytosis, a relatively mature field which could benefit greatly from updated methods, in order to prompt further explorations of their similarities and differences. The methods on autophagy allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes, while the methods devoted to phagocytosis provide researchers with several modern techniques for in vitro and in vivo studies of phagosomal organelles. Following the successful Methods in Molecular Biology™ series format, chapters include step-by-step laboratory protocols, lists of necessary materials, and tips for troubleshooting and avoiding known pitfalls. Comprehensive and forward-thinking, Autophagosome and Phagosome offers a valuable guide to both cellular processes while inciting researchers to explore the potentially important connections between the two


Autophagosome and Phagosome -- Fine Structure of the Autophagosome -- Methods for Assessing Autophagy and Autophagic Cell Death -- LC3 and Autophagy -- Amino Acid Regulation of Autophagosome Formation -- Autophagic Proteolysis of Long-Lived Proteins in Nonliver Cells -- Autophagosomes in GFP-LC3 Transgenic Mice -- Experimental Control and Characterization of Autophagy in Drosophila -- Analysis of Autophagosome Membrane Cycling by Fluorescence Microscopy -- Protein Trafficking into Autophagosomes -- Sphingolipids in Macroautophagy -- Molecular Links Between Autophagy and Apoptosis -- Clearance of Mutant Aggregate-Prone Proteins by Autophagy -- Localization and MHC Class II Presentation of Antigens Targeted for Macroautophagy -- Chaperone-Mediated Autophagy -- Microautophagy in the Yeast Saccharomyces cerevisiae -- EM Analysis of Phagosomes -- Analysis of Phosphoinositide Dynamics During Phagocytosis Using Genetically Encoded Fluorescent Biosensors -- In Vitro Phagosome–Endosome Fusion -- Real-Time Spectrofluorometric Assays for the Lumenal Environment of the Maturing Phagosome -- Maturation Changes in Purified Phagosomes -- Large-Scale Purification of Latex Bead Phagosomes from Mouse Macrophage Cell Lines and Subsequent Preparation for High-Throughput Quantitative Proteomics -- Class II MHC Antigen Processing in Phagosomes -- Analyzing Association of the Endoplasmic Reticulum with the Legionella pneumophila–Containing Vacuoles by Fluorescence Microscopy -- Fractionation of the Coxiella burnetii Parasitophorous Vacuole -- Bacterial Phagosome Acidification Within IFN-?-Activated Macrophages: Role of Host p47 Immunity-Related GTPases IRGs) -- SopE-Mediated Recruitment of Host Rab5 on Phagosomes Inhibits Salmonella Transport to Lysosomes -- The Mycobacterium tuberculosis Phagosome

Life sciences Molecular biology Biochemistry Cell biology Microbiology Life Sciences Biochemistry general Cell Biology Molecular Medicine Microbiology


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