XXII, 484 p. 89 illus., 6 illus. in color. online resource
SUMMARY
Rapid changes and significant progress have been made in the use of Agrobacterium to genetically transform plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Second Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail their best techniques for delivering DNA to plant cells and permanently altering their genomes. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction, major components of plant transformation that are often neglected by many plant biologists. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Agrobacterium Protocols, Second Edition, Volumes 1 and 2 offers plant biotechnologists a gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement, and for inspiration in developing new methods for other related and non-related plants
CONTENT
AGROBACTERIUM HANDLING -- Culture and Maintenance of Agrobacterium Strains -- Binary Vectors and Super-binary Vectors -- Three Methods for the Introduction of Foreign DNA into Agrobacterium -- Integration of Genes into the Chromosome of Agrobacterium tumefaciens C58 -- Nucleic Acid Extraction from Agrobacterium Strains -- Agrobacterium Virulence Gene Induction -- Model Plants -- Arabidopsis thaliana Floral Dip Transformation Method -- Agrobacterium Transformation of Arabidopsis thaliana Roots -- Medicago truncatula Transformation Using Leaf Explants -- Medicago truncatula Transformation Using Cotyledon Explants -- Medicago truncatula Transformation Using Root Explants -- Nicotiana (Nicotiana tobaccum, Nicotiana benthamiana) -- Generation of Composite Plants Using Agrobacterium rhizogenes -- Cereal Crops -- Barley (Hordeum vulgare L.) -- Maize (Zea mays L.) -- Indica Rice (Oryza sativa, BR29 and IR64) -- Japonica Rice Varieties (Oryza sativa, Nipponbare, and Others) -- Rye (Secale cereale L.) -- Sorghum (Sorghum bicolor L.) -- Wheat (Triticum aestivum L.) -- Industrial Plants -- Canola (Brassica napus L.) -- Cotton (Gossypium hirsutum L.) -- Indian Mustard [Brassica juncea (L.) Czern.] -- Sunflower (Helianthus annuus L.) -- Legume Plants -- Alfalfa (Medicago sativa L.) -- Chickpea (Cicer arietinum L.) -- Clovers (Trifolium spp.) -- Peas (Pisum sativum L.) -- Peanut (Arachis hypogaea L.) -- Pigeonpea (Cajanus cajn (L.) Millsp.) -- Red Clover (Trifolium pratense) -- Soybean (Glycine max) Transformation Using Mature Cotyledonary Node Explants -- Soybean (Glycine max) Transformation Using Immature Cotyledon Explants -- Tepary Bean (Phaseolus acutifolius) -- Vegetable Plants -- Brassica oleracea -- Cucumber (Cucumis sativus L.) -- Eggplant (Solanum melongena L.) -- Lettuce (Lactuca sativa L.) -- Tomato (Lycopersicum esculentum)
