Title | Redox-Mediated Signal Transduction [electronic resource] : Methods and Protocols / edited by John T. Hancock |
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Imprint | Totowa, NJ : Humana Press, 2009 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-129-1 |
Descript | X, 234 p. 48 illus., 2 illus. in color. online resource |
The Role of Redox in Signal Transduction -- The Measurement of Nitric Oxide and Its Metabolites in Biological Samples by Ozone-Based Chemiluminescence -- Detection and Measurement of Reactive Oxygen Intermediates in Mitochondria and Cells -- Redox-Sensitive Green Fluorescent Protein: Probes for Dynamic Intracellular Redox Responses. A Review -- Measuring Redox Changes In Vivo in Leaves: Prospects and Technical Challenges -- Imaging of Intracellular Hydrogen Peroxide Production with Hyper Upon Stimulation of Hela Cells with Egf -- Tools to Investigate ROS Sensitive Signalling Proteins -- Methods for Preparing Crystals of Reversibly Oxidized Proteins: Crystallization of Protein Tyrosine Phosphatase 1B as an Example -- Methods for the Study of Redox-Mediated Changes in p53 Structure and Function -- Redox Regulation and Trapping Sulphenic Acid in the Peroxide Sensitive Human Mitochondrial Branched Chain Aminotransferase -- Detection of Carbonylated Proteins in 2-D SDS Page Separations -- Analysis of Global and Specific Changes in the Disulfide Proteome Using Redox 2D-PAGE -- Protein-Thiol Oxidation, From Single Proteins to Proteome-Wide Analyses -- Analysis of Redox Relationships in the Plant Cell Cycle: Determinations of Ascorbate, Glutathione and Poly (ADPribose) Polymerase (PARP) in Plant Cell Cultures -- Generation and Detection of S-Nitrosothiols