Title | Osteoporosis [electronic resource] : Methods and Protocols / edited by Jennifer J. Westendorf |
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Imprint | Totowa, NJ : Humana Press, 2008 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-104-8 |
Descript | XIX, 354 p. online resource |
In Vitro Models -- Isolation and Culture of Rodent Osteoprogenitor Cells -- Osteoclast Culture and Resorption Assays -- Assessing New Bone Formation in Neonatal Calvarial Organ Cultures -- Detection of Apoptosis of Bone Cells In Vitro -- In Vivo Rodent Models -- In Vivo Parathyroid Hormone Treatments and RNA Isolation and Analysis -- Assessment of Bone Formation Capacity Using In vivo Transplantation Assays: Procedure and Tissue Analysis -- Ossicle and Vossicle Implant Model Systems -- Utility of the Ovariectomized Rat as a Model for Human Osteoporosis in Drug Discovery -- Orchidectomy Models of Osteoporosis -- Methods of Gene Delivery -- Gene Delivery by Adenoviruses -- Lentivirus Delivery of shRNA Constructs into Osteoblasts -- Gene Delivery by Retroviruses -- Molecular and Genetic Techniques -- Chromatin Immunoprecipitation Assays: Application of ChIP-on-Chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells -- Identification of Transcription Factor Target Genes by ChIP Display -- Application of the Laser Capture Microdissection Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo -- Quantitative Trait Loci Mapping -- Imaging Techniques -- In Situ Nuclear Organization of Regulatory Machinery -- Bioluminescent Imaging in Bone -- Micro-Computed Tomography: A Method for the Non-Destructive Evaluation of the Three-Dimensional Structure of Biological Specimens -- Fourier Transform-Infrared Microspectroscopy and Microscopic Imaging -- Mechanical Testing -- Assessment of the In Vivo Adaptive Response to Mechanical Loading -- Determination of Bone's Mechanical Matrix Properties by Nanoindentation -- Fluid Flow Assays