Title | Handbook of ELISPOT [electronic resource] : Methods and Protocols / edited by Alexander E. Kalyuzhny |
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Imprint | Totowa, NJ : Humana Press, 2005 |
Connect to | http://dx.doi.org/10.1385/1592599036 |
Descript | XIV, 322 p. 123 illus., 10 illus. in color. online resource |
Elispot Assay Setup and Data Analysis -- ELISPOT Assay -- Chemistry and Biology of the ELISPOT Assay -- Membranes and Membrane Plates Used in ELISPOT -- Standardization and Validation Issues of the ELISPOT Assay -- A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay -- Isolation of Subsets of Immune Cells -- Image Analysis and Data Management of ELISPOT Assay Results -- High Resolution as a Key Feature to Perform Accurate ELISPOT Measurements Using Zeiss KS ELISPOT Readers -- Elispot Assay in Animal Models -- Improving the Sensitivity of the ELISPOT Analyses of Antigen-Specific Cellular Immune Responses in Rhesus Macaques -- Feline Cytokine ELISPOT -- Measuring T-Cell Function in Animal Models of Tuberculosis by ELISPOT -- Interferon-? ELISPOT Assay for the Quantitative Measurement of Antigen-Specific Murine CD8+ T-Cells -- Elispot Assays in Human Studies -- Detection of Measles Virus-Specific Interferon-?-Secreting T-Cells by ELISPOT -- Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assay -- Use of Interferon-? ELISPOT in Monitoring Immune Responses in Humans -- ELISPOT Determination of Interferon-? T-Cell Frequencies in Patients With Autoimmune Sensorineural Hearing Loss -- Multiplex and Modified Elispot Assay Formats -- Dual-Color ELISPOT Assay for Analyzing Cytokine Balance -- Simultaneous Detection of Multiple Cytokines in ELISPOT Assays -- Fluorospot Assay -- A Gel-Based Dual Antibody Capture and Detection Method for Assaying of Extracellular Cytokine Secretion