Title	Laser Capture Microdissection [electronic resource] : Methods and Protocols / edited by Graeme I. Murray, Stephanie Curran
Imprint	Totowa, NJ : Humana Press, 2005
Connect to	http://dx.doi.org/10.1385/1592598536
Descript	XIV, 320 p. 82 illus., 1 illus. in color. online resource

Use of laser microdissection has grown enormously to become a very powerful high-throughput technology essential to a range of downstream applications in pathology, cancer research, life sciences, medical diagnostics, and biotechnology. In Laser Capture Microdissection, expert laboratory researchers noted for their optimized methods describe in step-by-step detail not only how to successfully carry out tissue-based laser microdissection in their own laboratories using the different systems available, but also how to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. These readily reproducible methods make it possible to carry out contamination-free molecular analyses of specific types of normal and diseased cells. Highlights include gene expression profiling of primary tumor cell populations, RNA transcript amplification and GeneChip® microarrays, colorectal cancer proteomics, and proteomic analysis of human bladder tissue using the SELDI® approach. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Laser Capture Microdissection provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems and to the interpretation of the results using a wide range of sophisticated molecular technologies

An Introduction to Laser-Based Tissue Microdissection Techniques -- Microdissection and DNA Analysis -- Methacarn Fixation for Genomic DNA Analysis in Microdissected Cells -- Multiplex Quantitative Real-Time PCR of Laser Microdissected Tissue -- comparative Genomic Hybridization Using DNA From Laser Capture Microdissected Tissue -- Detection of Ki-ras and p53 Mutations by Laser Capture Microdissection/PCR/SSCP -- Whole-Genome Allelotyping Using Laser Microdissected Tissue -- Microdissection for Detecting Genetic Aberrations in Early and Advanced Human Urinary Bladder Cancer -- Laser Microdissection for Microsatellite Analysis in Colon and Breast Cancer -- Assessment of RET/PTC Oncogene Activation in Thyroid Nodules Utilizing Laser Microdissection Followed by Nested RT-PCR -- Combined Laser-Assisted Microdissection and Short Tandem Repeat Analysis for Detection of In Situ Microchimerism After Solid Organ Transplantation Ulrich Lehmann, Anne Versmold, and Hans Kreipe -- RNA and Gene Expression Studies Using Microdissected Cells -- Laser-Assisted Microdissection of Membrane-Mounted Tissue Sections -- Laser-Assisted Microdissection of Membrane-Mounted Sections Following Immunohistochemistry and In Situ Hybridization -- Laser-Assisted Cell Microdissection Using the PALM System -- Laser Microdissection and RNA Analysis -- Gene Expression Profiling of Primary Tumor Cell Populations Using Laser Capture Microdissection, RNA Transcript Amplification, and GeneChip® Microarrays -- 16 Quantification of Gene Expression in Mouse and Human Renal Proximal Tubules -- Laser Capture Microdissection for Analysis of Macrophage Gene Expression From Atherosclerotic Lesions -- Analysis of Pituitary Cells by Laser Capture Microdissection -- Microdissection Techniques And Applications In Proteomics -- Laser Capture Microdissection and Colorectal Cancer Proteomics -- Proteomic Analysis of Human Bladder Tissue Using SELDI® Approach Following Microdissection Techniques -- Microdissection And Molecular Analysis Of Microorganisms -- Genetic Analysis of HIV by In Situ PCR-Directed Laser Capture Microscopy of Infected Cells -- Use of Laser Capture Microdissection Together With In Situ Hybridization and Real-Time PCR to Study Distribution of Latent Herpes Simplex Virus Genomes in Mouse Trigeminal Ganglia -- Laser Capture Microdissection and PCR for Analysis of Human Papilloma Virus Infection -- Laser Capture Microdissection of Hepatic Stages of the Human Parasite Plasmodium falciparum for Molecular Analysis

1. Life sciences
2. Biochemistry
3. Cell biology
4. Life Sciences
5. Biochemistry
6. general
7. Cell Biology