Title | DNA Viruses [electronic resource] : Methods and Protocols / edited by Paul M. Lieberman |
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Imprint | Totowa, NJ : Humana Press, 2005 |
Connect to | http://dx.doi.org/10.1385/159259848X |
Descript | XIV, 498 p. online resource |
Viral Detection -- Viral Detection -- Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1 -- Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR -- Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In Vivo -- Detection and Quantification of the Rare Latently Infected Cell Undergoing Herpes Simplex Virus Transcriptional Activation in the Nervous System In Vivo -- Reporter Cell Lines for the Detection of Herpes Simplex Viruses -- Virus Structure And Imaging -- Unraveling the Architecture of Viruses by High-Resolution Atomic Force Microscopy -- Studying the Structure of Large Viruses With Multiresolution Imaging -- Herpes Simplex Virus-Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence -- Herpes Simplex Virus-Cell Interactions Studied by Immunogold Cryosection Electron Microscopy -- FTIR Microscopy Detection of Cells Infected With Viruses -- Virus Entry -- The JC Virus-Like Particle Overlay Assay -- Analysis of Fusion Using a Virus-Free Cell Fusion Assay -- Pseudovirions as Specific Tools for Investigation of Virus Interactions With Cells -- Gene Expression -- Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence -- Identification and Characterization of Herpesviral Immediate-Early Genes -- Replication And Genome Maintenance -- Methods for Measuring the Replication and Segregation of Epstein-Barr Virus-Based Plasmids -- DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins -- Pathogenesis -- Pre-B-Cell Colony Formation Assay -- Luciferase Real-Time Bioluminescence Imaging for the Study of Viral Pathogenesis -- Complex Cell Systems -- Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection -- Human Papillomavirus Type 31 Life Cycle Methods for Study Using Tissue Culture Models -- Recombinant Genetics -- Molecular Genetics of Herpesviruses A Recombinant Technology Approach -- Molecular Genetics of DNA Viruses Recombinant Virus Technology -- Genetic Analysis of Cytomegalovirus by Shuttle Mutagenesis -- Construction of a Gene Inactivation Library for Bovine herpesvirus 1 Using Infectious Clone Technology -- Selective Silencing of Viral Gene E6 and E7 Expression in HPV-Positive Human Cervical Carcinoma Cells Using Small Interfering RNAs -- Computation/Systems Biology Of Viruses -- Design of a Herpes Simplex Virus Type 2 Long Oligonucleotide-Based Microarray -- Real-Time Quantitative PCR Analysis of Viral Transcription -- Rapid Screening of Chemical Inhibitors That Block Processive DNA Synthesis of Herpesviruses