The application of recombinant DNA technology to industrial fermentation and the production of enzymes over the last 20 years has produced a host of useful chemical and biochemical substances. In Microbial Enzymes and Biotransformations, leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Two overview chapters consider microorganisms as a source of metabolic and enzymatic diversity, and enzymes in the fast-moving field of enzyme biosensors. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Processes and Products, provides laboratory procedures for a wide range of processes and products mediated by microorganisms. Comprehensive and highly practical, Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications
CONTENT
Microbial Cells and Enzymes A Century of Progress -- Enzyme Biosensors -- Directed Evolution of Enzymes -- L-Glutaminase as a Therapeutic Enzyme of Microbial Origin -- Enzymatic Production of D-Amino Acids -- Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides -- Food-Grade Corynebacteria for Enzyme Production -- Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase -- Enzymes in Modern Detergents -- Microbial Proteases -- Proteases Produced by Halophilic Bacteria and Archaea -- Microbial Pectinases -- Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes -- Glucose Dehydrogenase for the Regeneration of NADPH and NADH -- Acetate Kinase From Methanosarcina thermophila, a Key Enzyme for Methanogenesis -- Immobilization of Enzymes by Covalent Attachment -- Preparation of an Industrial Biocatalyst of Penicillin G Acylase on Sepabeads -- Methods for Bioencapsulation of Enzymes and Cells
