Title | Synthetic Protein Switches [electronic resource] : Methods and Protocols / edited by Viktor Stein |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6940-1 |
Descript | XI, 341 p. 59 illus., 31 illus. in color. online resource |
Synthetic Protein Switches: Theoretical and Experimental Considerations -- Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange -- Construction of Protein Switches by Domain Insertion and Directed Evolution -- Catalytic Amyloid Fibrils that Bind Copper to Activate Oxygen -- Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors -- Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modelling and Assessment -- Rational Design and Applications of Semi-Synthetic Modular Biosensors: SNIFITs and LUCIDs -- Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases -- Quantitative and Dynamic Imaging of ATM Kinase Activity -- Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains -- Protein and Protease Sensing by Allosteric Derepression -- DNA-Specific Biosensors Based on Intramolecular β-Lactamase-Inhibitor Complex Formation -- Engineering and Characterizing Synthetic Protease Sensors and Switches -- Characterizing Dynamic Protein-Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV -- Development of a Synthetic Switch to Control Protein Stability in Eukaryotic Cells with Light -- Light-Regulated Protein Kinases Based on the CRY2-CIB1 System -- Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels -- Primer-Aided Truncation for the Creation of Hybrid Proteins -- Engineering Small Molecule Responsive Split-Protein Kinases -- Directed Evolution Methods to Rewire Signaling Networks