Title | The Immune Synapse [electronic resource] : Methods and Protocols / edited by Cosima T. Baldari, Michael L. Dustin |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6881-7 |
Descript | XIII, 588 p. 114 illus., 89 illus. in color. online resource |
The Immune Synapse: Past, Present, and Future -- Analyzing Actin Dynamics of the Immunological Synapse -- Analysis of Microtubules and Microtubule-Organizing Centre at the Immune Synapse -- Analyzing the Dynamics of Signaling Microclusters -- Reconstitution of TCR Signaling using Supported Lipid Bilayers -- Plasma Membrane Sheets for Studies of B Cell Antigen Internalization From Immune Synapses -- Studying The Dynamics Of TCR Internalization At The Immune Synapse -- T Cell Receptor Activation of NF-Κb in Effector T Cells: Visualizing Signaling Events Within and Beyond the Cytoplasmic Domain of the Immunological Synapse -- Imaging Vesicular Traffic at the Immune Synapse -- Analysis of TCR Recycling at the Immune Synapse -- Simultaneous Membrane Capacitance Measurements and TIRF Microscopy to Study Granule Trafficking at Immune Synapses -- Mathematical Modeling of Synaptic Patterns -- Super-Resolution Analysis of TCR-Dependent Signaling: Single Molecule Localization Microscopy -- Förster Resonance Energy Transfer to Study TCR-pMHC Interactions in the Immunological Synapse -- Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes -- Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains during Immunological Synapse Formation using Donor-Quenching FRET and FLIM-FRET -- Revealing the Role of Microscale Architecture in Immune Synapse Function through Surface Micropatterning -- Spatial Control of Biological Ligands on Surfaces -- Probing Synaptic Biomechanics using Micropillar Arrays -- Microchannels for Study of Immunological Synapses and Kinapses -- Purification of LAT Containing Membranes from Resting and Activated T Lymphocytes -- Quantitative Phosphoproteomic Analysis of T-Cell Receptor Signaling -- Imaging Asymmetric T Cell Division -- Ultrastructure Of Immune Synapses -- Systems Imaging of the Immune Synapse -- Comprehensive Analysis of Immunological Synapse Phenotypes using Supported Lipid Bilayers -- Studying Immunoreceptor Signaling in Human T Cells using Electroporation of In Vitro Transcribed mRNA -- A Protein Expression Tool-Kit for Studying Signalling in T Cells -- Imaging The Effector CD8 Synapse -- The Mast Cell Antibody-Dependent Degranulatory Synapse -- Measurement of Lytic Granule Convergence after Formation of an NK Cell Immunological Synapse -- Studying the T Cell-Astrocyte Immune Synapse -- Aberrant Immunological Synapses Driven by Leukemic Antigen Presenting Cells -- Studying the Immune Synapse in HIV-1 Infection -- In Vivo Imaging of T Cell Immunological Synapses and Kinapses in Lymph Nodes -- Studying Dendritic Cell-T Cell Interactions Under In Vivo Conditions.