Title | Plant Pattern Recognition Receptors [electronic resource] : Methods and Protocols / edited by Libo Shan, Ping He |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6859-6 |
Descript | XIV, 358 p. 57 illus., 39 illus. in color. online resource |
Peptidoglycan Isolation and Binding Studies with LysM-Type Pattern Recognition Receptors -- Characterization of Plant Cell Wall Damage-associated Molecular Patterns Regulating Immune Responses -- Methods of Isolation and Characterization of Oligogalacturonide Elicitors -- Quantitative Analysis of Ligand-induced Endocytosis of FLAGELLIN-SENSING 2 using Automated Image Segmentation -- Analysis for Protein Glycosylation of Pattern Recognition Receptors in Plants -- Assays to Investigate the N-glycosylation State and Function of Plant Pattern Recognition Receptors -- Steady-State and Kinetics-Based Affinity Determination in Effector-Effector Target Interactions -- In Vitro Ubiquitination Activity Assays in Plant Immune Responses -- Bioinformatics Analysis of the Receptor-Like Kinase (RLK) Superfamily -- Identification of MAPK Substrates using Quantitative Phosphoproteomics -- Analysis of PAMP-Triggered ROS Burst in Plant Immunity -- LeEIX2 interactors’ Analysis and EIX-mediated Response Measurement -- MAPK Assays in MAMP-PRR Signal Transduction Pathway in Arabidopsis -- CDPK Activation in PRR Signaling -- Protein Kinase Assays -- Measuring Callose Deposition, an Indicator of Cell Wall Reinforcement, during Bacterial Infection in Arabidopsis -- Quantitative Evaluation of Plant Actin Cytoskeletal Organization during Immune Signaling -- Network Reconstitution for Quantitative Subnetwork Interaction Analysis -- Stomatal Bioassay to Characterize Bacterial-stimulated PTI at the Pre-invasion Phase of Infection -- Using Clear Nail Polish to Make Arabidopsis Epidermal Impressions for Measuring the Change of Stomatal Aperture Size in Immune Response -- Characterizing the Immune-Eliciting Activity of Putative Microbe-Associated Molecular Patterns in Tomato -- Genome-Wide Analysis of Chromatin Accessibility in Arabidopsis Infected with Pseudomonas syringae -- Small RNA and mRNA Profiling of Arabidopsis Infected by Phytophthora -- Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants -- Rapid Construction of Multiplexed CRISPR-Cas9 Systems for Plant Genome Editing -- Chitin-triggered MAPK activation and ROS generation in Rice Suspension-cultured Cells -- Chitin Induced Responses in the Moss Physcomitrella patens -- Methods to Quantify PAMP-Triggered MAP Kinase Phosphorylation, Oxidative Burst, and Lignification in Brassicas -- Effectoromics-Based Identification of Cell Surface Receptors in Potato.