Title | Protein Terminal Profiling [electronic resource] : Methods and Protocols / edited by Oliver Schilling |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6850-3 |
Descript | XII, 268 p. 53 illus., 37 illus. in color. online resource |
[14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay -- DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity -- SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation -- Profiling of Protein N-Termini and their Modifications in Complex Samples -- Protease Substrate Profiling by N-Terminal COFRADIC -- Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification -- Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types -- System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates -- Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC -- ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling -- Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP) -- Multiplexed Protease Specificity Profiling Using Isobaric Labeling -- FPPS - Fast Profiling of Protease Specificity -- Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics -- Prediction of Proteases Involved in Peptide Generation -- Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches -- Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors -- Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method -- Synthesis and Application of Activity-Based Probes for Proteases