Title | Exosomes and Microvesicles [electronic resource] : Methods and Protocols / edited by Andrew F Hill |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6728-5 |
Descript | XII, 228 p. 39 illus., 21 illus. in color. online resource |
Methods to Analyze EVs -- Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles -- Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis -- Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy -- Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR -- Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ System -- A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration -- Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array) -- Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation -- A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space -- Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission -- Isolation of Platelet-Derived Extracellular Vesicles -- Bioinformatics Tools for Extracellular Vesicles Research -- Preparation and Isolation of siRNA-Loaded Extracellular Vesicles -- Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions