Title | Metagenomics [electronic resource] : Methods and Protocols / edited by Wolfgang R. Streit, Rolf Daniel |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Edition | 2nd ed. 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6691-2 |
Descript | XI, 303 p. 48 illus., 24 illus. in color. online resource |
Construction of Small-Insert and Large-Insert Metagenomic Libraries -- Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies -- Construction and Screening Marine Metagenomic Large Insert Libraries -- Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment -- DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms -- Assessing Bacterial and Fungal Diversity in the Plant’s Endosphere -- Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities -- Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production -- Degradation Network Reconstruction Guided by Metagenomic Data -- Novel Tools for the Functional Expression of Metagenomics DNA -- A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries -- Screening for Cellulase Encoding Clones in Metagenomics Libraries -- Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes -- Screening Glycosyltransferases for Polyphenol Modifications -- Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial Communities -- Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity -- Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes -- Screening for N-AHSL-Based-Signaling Interfering Enzymes -- Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites