Title | Epstein Barr Virus [electronic resource] : Methods and Protocols / edited by Janos Minarovits, Hans Helmut Niller |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6655-4 |
Descript | XIV, 269 p. 22 illus., 21 illus. in color. online resource |
Current Trends and Alternative Scenarios in EBV Research -- Epstein-Barr Virus: Clinical Diagnostics -- Establishment of EBV-Infected Lymphoblastoid Cell Lines -- Generation and Infection of Organotypic Cultures with Epstein-Barr Virus -- Affinity Purification-Mass Spectroscopy Methods for Identifying Epstein-Barr Virus-Host Interactions -- The Use of 3D Telomere FISH for the Characterization of the Nuclear Architecture in EBV-Positive Hodgkin’s Lymphoma -- Analysis of EBV Transcription Using High-Throughput RNA Sequencing -- Analysis of Viral Promoter Usage in EBV-Infected Cell Lines: A Comparison of qPCR following Conventional RNA Isolation and Nuclear Run-On Assay -- Analysis of Viral and Cellular MicroRNAs in EBV Infected Cells -- Isolation and Characterization of Exosomes Released by EBV Immortalized Cells -- Functional Analysis of Exosomes Derived from EBV-Infected Cells -- Terminal Repeat Analysis of EBV Genomes -- Characterization of EBV Promoters and Coding Regions by Sequencing PCR-Amplified DNA Fragments -- The Use of Chromatin Precipitation Coupled to DNA Sequencing (ChIP-Seq) for the Analysis of Zta Binding to the Human and EBV Genome -- Analysis of Viral Epigenotypes Using Bisulfite Sequencing: A Detailed Protocol for the Crucial Bisulfite Modification and PCR Amplification Steps -- Analysis of Viral Epigenotypes using Chromatin Immunoprecipitation -- Mice with Reconstituted Human Immune System Components as a Tool to Study Immune Cell Interactions in EBV Infection -- Generation and Analysis of Humanized Mouse Model of EBV Infection -- EBV-Directed T Cell Therapeutics for EBV-Associated Lymphomas