Title | Drug Target miRNA [electronic resource] : Methods and Protocols / edited by Marco F. Schmidt |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6563-2 |
Descript | XI, 319 p. 94 illus., 62 illus. in color. online resource |
MiRNA Targeting Drugs: The Next Blockbusters? -- Functional Analysis of miRNA using DIANA Tools Online Suite -- Non-Nucleotide Modification of Anti-miRNA Oligonucleotides -- Quantification of Oligonucleotide Association with miRNA-Argonaute Complexes In Vitro -- Determination of Anti-miR Association with miRNA/Argonaute Complexes In Vivo -- Competitive Argonaute-Based RNA Immunoprecipitation for Investigation of Transcriptomic Response to Anti-miR -- Assessing Anti-miR Pharmacology with miRNA Polysome Shift Assay -- Evaluating Synergistic Effects of miR-34a Mimics in Combination with Other Therapeutic Agents in Cultured Non-Small Cell Lung Cancer Cells -- Assessing the Off-Target Effects of miRNA Inhibitors on Innate Immune Toll-Like Receptors -- Design of Multimodal Small Molecules Targeting miRNA Biogenesis: Synthesis and In Vitro Evaluation -- Machine Learning Approaches towards Building Predictive Models for Small Molecule Modulators of miRNA and its Utility in Virtual Screening of Molecular Databases -- Identification of Small Molecule Modulators of MicroRNA by Library Screening -- Rapid Generation of miRNA Inhibitor Leads by Bio-Informatics and Efficient High-Throughput Screening Methods -- Peptide-Based Inhibition of miRNA-Guided Gene Silencing -- Small Molecules Targeting the miRNA Binding Domain of Argonaute 2: From Computer-Aided Molecular Design to RNA Immunoprecipitation -- Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule Argonaute 2 Protein Binders -- Antagonists of the miRNA-Argonaute 2 Protein Complex: Anti-miR-AGOs -- Elucidating Mechanisms of Molecular Recognition between Human Argonaute and miRNA using Computational Approaches -- Kinetic Analysis of Target RNA Binding and Slicing by Argonaute 2 Protein -- Site-Specific Fluorescent Labeling of Argonaute for FRET-Based Bio-Assays -- Single-Molecule Fluorescence Energy Transfer Assays for the Characterization of Reaction Pathways of miRNA-Argonaute Complex