Title | T-Cell Differentiation [electronic resource] : Methods and Protocols / edited by Enrico Lugli |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6548-9 |
Descript | XI, 271 p. 55 illus., 44 illus. in color. online resource |
The Vast Universe of T Cell Diversity: Subsets of Memory Cells and their Differentiation -- Basic Aspects of T Helper Cell Differentiation -- FACS Analysis of Memory T lymphocytes -- Intravital Microscopy Analysis of Hepatic T cell dynamics -- Analysis of T Cell Activation by Confocal Microscopy -- Phenotypic and Functional Analysis of Antigen-specific T Cell Exhaustion -- pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel -- Differentiation of Diverse Progenies of Memory T cells from Naïve CD8+ T Cell Precursors -- Gammaretroviral Production and T Cell Transduction To Genetically Retarget Primary T Cells Against Cancer -- Measuring Telomerase Activity in Senescent Human T Cells upon Genetic Modification -- Strategies for T Helper Cell Subset Differentiation from Naïve Precursors -- Phenotypic and Functional Analysis of the Suppressive Function of Human Regulatory T Cells -- Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation -- Next-generation Sequencing Analysis of Long Non-Coding RNAs in CD4+ T Cell Differentiation -- Highly Multiplexed, Single Cell Transcriptomic Analysis of T-cells by Microfluidic PCR -- Single-cell RNA Sequencing of Human T Cells -- Extensive Phenotypic Analysis, Transcription Factor Profiling, and Effector Cytokine Production of Human MAIT Cells by Flow Cytometry -- Developmental and Functional Assays to Study Murine and Human gd T Cells.