|Title||T-Cell Differentiation [electronic resource] : Methods and Protocols|
|Author||edited by Enrico Lugli|
|Imprint||New York, NY : Springer New York : Imprint: Humana Press, 2017|
|Descript||XI, 271 p. 55 illus., 44 illus. in color. online resource|
The Vast Universe of T Cell Diversity: Subsets of Memory Cells and their Differentiation -- Basic Aspects of T Helper Cell Differentiation -- FACS Analysis of Memory T lymphocytes -- Intravital Microscopy Analysis of Hepatic T cell dynamics -- Analysis of T Cell Activation by Confocal Microscopy -- Phenotypic and Functional Analysis of Antigen-specific T Cell Exhaustion -- pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel -- Differentiation of Diverse Progenies of Memory T cells from Naïve CD8+ T Cell Precursors -- Gammaretroviral Production and T Cell Transduction To Genetically Retarget Primary T Cells Against Cancer -- Measuring Telomerase Activity in Senescent Human T Cells upon Genetic Modification -- Strategies for T Helper Cell Subset Differentiation from Naïve Precursors -- Phenotypic and Functional Analysis of the Suppressive Function of Human Regulatory T Cells -- Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation -- Next-generation Sequencing Analysis of Long Non-Coding RNAs in CD4+ T Cell Differentiation -- Highly Multiplexed, Single Cell Transcriptomic Analysis of T-cells by Microfluidic PCR -- Single-cell RNA Sequencing of Human T Cells -- Extensive Phenotypic Analysis, Transcription Factor Profiling, and Effector Cytokine Production of Human MAIT Cells by Flow Cytometry -- Developmental and Functional Assays to Study Murine and Human gd T Cells.
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