Title | High-Throughput Glycomics and Glycoproteomics [electronic resource] : Methods and Protocols / edited by Gordan Lauc, Manfred Wuhrer |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6493-2 |
Descript | XI, 274 p. 51 illus., 42 illus. in color. online resource |
Ubiquitous Importance of Protein Glycosylation -- Laboratory Experimental Design for a Glycomic Study -- High-Throughput Analysis of the IgG N-Glycome by UPLC-FLR -- High-Throughput Analysis of IgG Fc Glycopeptides by LC-MS -- Sialic Acid Derivatization for the Rapid Subclass and Sialic Acid Linkage-Specific MALDI-TOF-MS Analysis of IgG Fc-Glycopeptides -- The Use of Multiple Reaction Monitoring on QQQ-MS for the Analysis of Protein- and Site-Specific Glycosylation Patterns in Serum -- Analysis of Permethylated Glycan by Liquid Chromatography (LC) and Mass Spectrometry (MS) -- High-Throughput Analysis of the Plasma N-Glycome by UHPLC -- Site-Specific N- and O-Glycopeptide Analysis Using an Integrated C18-PGC-LC-ESI-QTOF-MS/MS Approach -- Analysis of Milk Oligosaccharides by Mass Spectrometry -- N- and O-Glycomics from Minor Amounts of Formalin-Fixed, Paraffin-Embedded Tissue Samples -- Carbohydrate Microarray Technology Applied to High-Throughput Mapping of Plant Cell Wall Glycans Using Comprehensive Microarray Polymer Profiling (CoMPP) -- Analysis of Invertebrate and Protist N-Glycans -- High-Throughput and High-Sensitivity Mass Spectrometry-Based N-Glycomics of Mammalian Cells -- Analysis of N-Glycosylation of Total Membrane Proteins -- HILIC-UPLC Analysis of Brain Tissue N-Glycans -- Automated Integration of a UPLC Glycomic Profile -- Databases and Associated Tools for Glycomics and Glycoproteomics -- High-Throughput N-Glycan Analysis with Rapid Magnetic Bead-Based Sample Preparation.