Title | Plant Hormones [electronic resource] : Methods and Protocols / edited by Jürgen Kleine-Vehn, Michael Sauer |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Edition | 3rd ed. 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6469-7 |
Descript | XI, 288 p. 117 illus., 106 illus. in color. online resource |
Real Time Analysis of the Apical Hook Development -- Grafting with Arabidopsis thaliana -- Tips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants -- Assaying Germination and Seedling Responses of Arabidopsis to Karrikins -- Low-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping -- Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research -- High throughput scoring of Seed Germination -- Histochemical Staining of β-Glucuronidase and its Spatial Quantification -- Imaging TCSn::GFP, A Synthetic Cytokinin Reporter, in Arabidopsis thaliana -- Highlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins -- In-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas -- Analysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin -- FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells -- In vivo Identification of Plant Protein Complexes using IP-MS/MS -- Assaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons -- Studying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP) -- Hormone Receptor Glycosylation -- Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue -- High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana -- Hormone Profiling in Plant Tissues -- Use of Xenopus laevis Oocytes to Study Auxin Transport -- Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.