Title | Wnt Signaling [electronic resource] : Methods and Protocols / edited by Quinn Barrett, Lawrence Lum |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2016 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6393-5 |
Descript | XI, 198 p. 45 illus., 32 illus. in color. online resource |
Visualizing Wnt Palmiteoylation in Single Cells -- Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction -- Biochemical Methods to Analyze Wnt Protein Secretion -- Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum -- Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway -- A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in Cells -- Wnt-Dependent Control of Cell Polarity in Cultured Cells -- The Use of Chick Embryos to Study Wnt Signaling/Gradients -- Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors -- Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo -- Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts -- Delivery of the Porcupine Inhibitor WNT974 in Mice -- Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis -- Monitoring Wnt/b-Catenin Signaling in Skin -- The Generation of Organoids for Studying Wnt Signaling -- Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells -- Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions