Title | Clostridium difficile [electronic resource] : Methods and Protocols / edited by Adam P. Roberts, Peter Mullany |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2016 |
Edition | 2nd ed. 2016 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6361-4 |
Descript | XI, 292 p. 49 illus., 33 illus. in color. online resource |
Restriction Endonuclease Analysis Typing of Clostridium Difficile Isolates -- Direct PCR-ribotyping of Clostridium difficile -- From FASTQ to Function: In silico Methods for Processing Next Generation Sequencing Data -- Clostridium difficile Genome Editing Using pyrE Alleles -- Use of mCherryOpt Fluorescent Protein in Clostridium difficile -- A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile -- Clostridium difficile Adhesins -- Intestinal Epithelial Cell Response to Clostridium difficile Flagella -- Isolating and Purifying Clostridium difficile Spores -- Inducing and Quantifying Clostridium difficile Spore Formation -- Characterization of Functional Prophages in Clostridium difficile -- Induction and Purification of C. difficile Phage Tail-like Particles -- Phage Transduction -- Transfer of Clostridium difficile Genetic Elements Conferring Resistance to Macrolide-Lincosamide-streptograminB (MLSB) Antibiotics -- Methods for Determining Transfer of Mobile Genetic Elements in Clostridium difficile -- Investigating Transfer of Large Chromosomal Regions Containing the Pathogenicity Locus between Clostridium difficile Strains -- An in vitro Model of the Human Colon: Studies of Intestinal Biofilms and lostridium difficile Infection -- MiniBioReactor Arrays (MBRAs) as a Tool for Studying C. difficile Physiology in the Presence of a Complex Community -- A Practical Method for Preparation of Fecal Microbiota Transplantation -- Ion Exchange Chromatography to Analyze Components of a Clostridium difficile Vaccine -- A Size Exclusion Chromatography Method for Analysis of Clostridium difficile Vaccine Toxins