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TitleApoptosis Methods in Toxicology [electronic resource]
Author edited by Perpetua M. Muganda
ImprintNew York, NY : Springer New York : Imprint: Humana Press, 2016
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Descript X, 244 p. 46 illus., 35 illus. in color. online resource


This detailed volume provides a single, valuable reference source for methods that definitively identify and accurately quantify apoptosis. The book begins with common methods utilized to detect and quantitate apoptosis, as well as apoptosis signaling pathways in toxicological and other related research. It continues with multi-parametric and phased apoptosis assays for detecting early and late apoptosis or distinguishing apoptosis from necrosis and autophagy. Subsequent chapters focus on recent advances in real time and high-throughput assays that detect and quantitate apoptosis and apoptosis signaling pathways. Final chapters focus on recent developments in preclinical anticancer therapeutics targeting apoptosis. Written for the Methods in Pharmacology and Toxicology series, chapters feature step-by-step descriptions of the methodologies, as well as expert tips and implementation advice. Vital and authoritative, Apoptosis Methods in Toxicology serves novice scientists as well as experts, utilizing a range of instruments from common laboratory equipment to high-end expensive and automated machinery capable of performing real time apoptotic measurements


An Overview of Apoptosis Methods in Toxicological Research: Recent Updates -- Detection of Apoptosis: From Bench Side to Clinical Practice -- Determining the Extent of Toxicant-Induced Apoptosis Using Concurrent Phased Apoptosis Assays -- Measurement of Apoptosis by Multiparametric Flow Cytometry -- Detecting Apoptosis, Autophagy, and Necrosis -- A Low-Cost Method for Tracking the Induction of Apoptosis Using FRET-Based Activity Sensors in Suspension Cells -- FRET-Based Measurement of Apoptotic Caspase Activities by High-Throughput Screening Flow Cytometry -- Automated Ratio Imaging Using Nuclear-Targeted Fret Probe-Expressing Cells for Apoptosis Detection -- Antibody-Based Proteomic Analysis of Apoptosis Signaling -- Novel Electrochemical Biosensor for Apoptosis Evaluation -- Microplate-Based Whole Zebrafish Caspase 3/7 Assay for Screening Small Molecule Compounds -- Targeting Cancer Cell Death with Small-Molecule Agents for Potential Therapeutics -- Liposomes in Apoptosis Induction and Cancer Therapy

Medicine Pharmacology Cell cycle Apoptosis Biomedicine Pharmacology/Toxicology Cell Cycle Analysis Apoptosis


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