Title | Programmed Cell Death [electronic resource] : Methods and Protocols / edited by Hamsa Puthalakath, Christine J. Hawkins |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2016 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-3581-9 |
Descript | XI, 291 p. 59 illus., 39 illus. in color. online resource |
Detection of Apoptotic Versus Autophagic Cell Death by Flow Cytometry -- In Vivo Apoptosis Imaging Using Site-Specifically 68Ga-Labeled Annexin V -- Detection of Active Caspases during Apoptosis Using Fluorescent Activity-Based Probes -- Detection of Initiator Caspase Induced Proximity in Single Cells by Caspase Bimolecular Fluorescence Complementation -- In Vitro Use of Peptide Based Substrates and Inhibitors of Apoptotic Caspases -- Experimental In Vivo Sepsis Models to Monitor Immune Cell Apoptosis and Survival in Laboratory Mice -- Analysis of Cell Death Induction in Intestinal Organoids In Vitro -- In Vitro Differentiation of Mouse Granulocytes -- Hydrodynamic Injection as a Method of Gene Delivery in Mice: A Model of Chronic Hepatitis B Virus Infection -- Isolation of Cardiomyocytes and Cardiofibroblasts for Ex-Vivo Analysis -- Detection of Cell Death in Drosophila Tissues -- Methods to Study Plant Programmed Cell Death -- Modeling Metazoan Apoptotic Pathways in Yeast -- Characterizing Bcl-2 Family Protein Conformation and Oligomerization Using Cross-Linking and Antibody Gel-Shift in Conjunction with Native PAGE -- Using Förster-Resonance Energy Transfer to Measure Protein Interactions between Bcl-2 Family Proteins on Mitochondrial Membranes -- Preparing Samples for Crystallization of Bcl-2 Family Complexes -- Screening Strategies for TALEN Mediated Gene Disruption -- Using CRISPR/Cas9 Technology for Manipulating Cell Death Regulators -- Lentiviral Vectors to Analyze Cell Death Regulators -- Proteomic Profiling of Cell Death: Stable Isotope Labeling and Mass Spectrometry Analysis.