Title | Chemotaxis [electronic resource] : Methods and Protocols / edited by Tian Jin, Dale Hereld |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2016 |
Edition | 2nd ed. 2016 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-3480-5 |
Descript | XIV, 425 p. 118 illus., 83 illus. in color. online resource |
In Situ Assays of Chemotropism during Yeast Mating -- Imaging Polarization in Budding Yeast Migration of Dictyostelium discoideum to the Chemoattractant Folic Acid -- Mitochondrial Stress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideum Cells -- Studying Chemoattractant Signal Transduction Dynamics in Dictyostelium by BRET -- Wave Patterns in Cell Membrane and Actin Cortex Uncoupled from Chemotactic Signals -- Chemotactic Blebbing in Dictyostelium Cells -- Dissecting Spatial and Temporal Sensing in Dictyostelium Chemotaxis Using a Wave Gradient Generator -- Employing Dictyostelium as an Advantageous 3Rs Model for Pharmacogenetic Research -- Identification of Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis -- Biochemical Responses to Chemically Distinct Chemoattractants during the Growth and Development of Dictyostelium -- Live Imaging of Border Cell Migration in Drosophila -- ShRNA-Induced Gene Knock-Down In Vivo to Investigate Neutrophil Function -- Studying Neutrophil Migration In Vivo Using Adoptive Cell Transfer -- Intravital Two-Photon Imaging of Lymphocytes Crossing High Endothelial Venules and Cortical Lymphatics in the Inguinal Lymph Node -- Flow Cytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response -- Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D -- 4D Tumorigenesis Model for Quantitating Coalescence, Directed Cell Motility and Chemotaxis, Identifying Unique Cell Behaviors and Testing Anti-Cancer Drugs -- An Experimental Model for Simultaneous Study of Migration of Cell Fragments, Single Cells and Cell Sheets -- Axon Guidance Studies Using a Microfluidics-Based Chemotropic Gradient Generator -- Visualization of Actin Assembly and Filament Turnover by In Vitro Multi-Color TIRF Microscopy -- Quantitative Monitoring Spatiotemporal Activation of Ras and PKD1 Using Confocal Fluorescent Microscopy -- Fluorescence Readout of a Patch Clamped Membrane by Laser Scanning Microscopy -- Use of Resonance Energy Transfer Techniques for In Vivo Detection of Chemokine Receptor Oligomerization -- Multi-State Transition Kinetics of Intracellular Signaling Molecules by Single-Molecule Imaging Analysis -- Mathematics of Experimentally Generated Chemoattractant Gradients -- Modeling Excitable Dynamics of Chemotactic Networks