Title | Poliovirus [electronic resource] : Methods and Protocols / edited by Javier Martín |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2016 |
Edition | 1st ed. 2016 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-3292-4 |
Descript | XI, 341 p. 75 illus., 58 illus. in color. online resource |
An Introduction to Poliovirus: Pathogenesis, Vaccination and the Endgame for Global Eradication -- Poliovirus Laboratory Based Surveillance: An Overview -- Isolation and Characterisation of Enteroviruses from Clinical Samples -- Isolation and Characterisation of Poliovirus in Cell Culture Systems -- Molecular Characterization of Polio from Environmental Samples – ISSP, The Israeli Sewage Surveillance Protocol -- Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays -- A Transgenic Mouse Model of Poliomyelitis -- Standardized Methods for Detection of Poliovirus Antibodies -- Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis,Typing by PCR and Real-time RT-PCR -- Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative -- Phylogenetic Analysis of Poliovirus Sequences -- Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA -- A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses -- Methods to Monitor Molecular Consistency of Oral Polio Vaccine -- Methods for the Quality Control of Inactivated Poliovirus Vaccines -- Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays -- Identification and Analysis of Antiviral Compounds against Poliovirus