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TitlePoliovirus [electronic resource] : Methods and Protocols
Author edited by Javier Martín
ImprintNew York, NY : Springer New York : Imprint: Humana Press, 2016
Edition 1st ed. 2016
Connect tohttp://dx.doi.org/10.1007/978-1-4939-3292-4
Descript XI, 341 p. 75 illus., 58 illus. in color. online resource

SUMMARY

This volume introduces the reader to the field of systems medicine by defining its terminology, and describing how established computational methods from bioinformatics and systems biology can be taken forward to establish integrative systems medicine approaches. Systems Medicine provides an outlook on the role that systems medicine may or should play in various medical fields. Describing different facets of the systems medicine approach in action, including examples related to human metabolism, chronic obstructive pulmonary disease, the RNAs in biomedicine and regenerative stem cell medicine. Chapters also introduce tools, resources and methodologies from bioinformatics and systems biology, and shows how to apply these in a systems medicine project. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, and discuss experimental and computational approaches, methods, and tools that should be considered for a successful systems medicine project. Systems Medicine aims to motivate and provide guidance for collaborations across disciplines to tackle today's challenges related to human health and well-being


CONTENT

An Introduction to Poliovirus: Pathogenesis, Vaccination and the Endgame for Global Eradication -- Poliovirus Laboratory Based Surveillance: An Overview -- Isolation and Characterisation of Enteroviruses from Clinical Samples -- Isolation and Characterisation of Poliovirus in Cell Culture Systems -- Molecular Characterization of Polio from Environmental Samples – ISSP, The Israeli Sewage Surveillance Protocol -- Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays -- A Transgenic Mouse Model of Poliomyelitis -- Standardized Methods for Detection of Poliovirus Antibodies -- Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis,Typing by PCR and Real-time RT-PCR -- Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative -- Phylogenetic Analysis of Poliovirus Sequences -- Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA -- A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses -- Methods to Monitor Molecular Consistency of Oral Polio Vaccine -- Methods for the Quality Control of Inactivated Poliovirus Vaccines -- Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays -- Identification and Analysis of Antiviral Compounds against Poliovirus


Medicine Virology Infectious diseases Medicine & Public Health Infectious Diseases Virology



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