Title | Recombinant Proteins from Plants [electronic resource] : Methods and Protocols / edited by Jacqueline MacDonald, Igor Kolotilin, Rima Menassa |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2016 |
Edition | 2nd ed. 2016 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-3289-4 |
Descript | XI, 296 p. 64 illus., 33 illus. in color. online resource |
Chloroplast Based Expression of Recombinant Proteins by Gateway® Cloning Technology -- Multigene Engineering in Rice Using High-Capacity Agrobacterium Tumefaciens BIBAC Vectors -- Virus-Derived Vectors for the Expression of Multiple Proteins in Plants -- Transient Protein Expression by Agroinfiltration In Lettuce -- Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas Reinhardtii -- Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes -- Transient Expression of Mammalian Genes In Nicotiana Benthamiana To Modulate N-Glycosylation -- Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants -- Production of Recombinant Cholera Toxin B Subunit in Nicotiana Benthamiana Using GENEWARE® Tobacco Mosaic Virus Vector -- Total Soluble Protein Extraction for Improved Proteomic Analysis Of Transgenic Plant Roots -- Temporary Immersion Bioreactors for the Contained Production Of Recombinant Proteins In Transplastomic Plants -- Plant Cell-Based Recombinant Antibody Manufacturing with A 200 L Orbitally Shaken Disposable Bioreactor -- Liquid-Liquid Phase Separation of Oil Bodies From Seeds -- Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture Extract -- Molecular Analyses of Transgenic Plants -- Analysis of Recombinant Proteins In Transgenic Rice Seeds: Identity, Localization, Tolerance To Digestion, And Plant Stress Response -- Real-Time PCR-Based Quantitation Method for The Genetically Modified Soybean Line GTS 40-3-2 -- A Standardized Lepidopteran Bioassay to Investigate the Bioactivity Of Insecticidal Proteins Produced In Transgenic Crops -- Generating Transgenic Plants Showing Antibody-Mediated Pathogen Resistance.